Identification of metabolites produced by Phanerochaete chrysosporium in the presence of amlodipine orotate using metabolomics.
Identifieur interne : 000261 ( Main/Exploration ); précédent : 000260; suivant : 000262Identification of metabolites produced by Phanerochaete chrysosporium in the presence of amlodipine orotate using metabolomics.
Auteurs : R B De Sotto [Corée du Sud] ; K I Kim [Corée du Sud] ; S. Kim [Corée du Sud] ; K G Song [Corée du Sud] ; Y. Park [Corée du Sud]Source :
- Water science and technology : a journal of the International Association on Water Pollution Research [ 0273-1223 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Amlodipine, Polluants chimiques de l'eau.
- métabolisme : Phanerochaete.
- méthodes : Chromatographie en phase liquide, Spectrométrie de masse.
- Dépollution biologique de l'environnement, Métabolome, Métabolomique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Amlodipine, Water Pollutants, Chemical.
- metabolism : Phanerochaete.
- methods : Chromatography, Liquid, Mass Spectrometry.
- Biodegradation, Environmental, Metabolome, Metabolomics.
Abstract
Pharmaceuticals are very useful in treating human diseases but they are excreted to the environment sometimes in their original form or as byproducts of human metabolism. Pharmaceuticals and their metabolites have been proven by studies to be harmful to non-target ecological species and may be persistent in different water matrices. In this regard, there is an emergent need to eliminate these compounds to prevent their adverse effects on aquatic species. Biodegradation using white-rot fungi is a promising technology for the removal of recalcitrant compounds; however, products of fungal biodegradation can also be detrimental. In this novel study, we evaluated the ability of Phanerochaete chrysosporium to degrade amlodipine, an anti-hypertensive drug which was recently found in water systems. Analysis of amlodipine metabolites was done using quadrupole time-of-flight liquid chromatography mass spectrometry after the degradation set-up of 120 hours. Pharmaceutical degradation was seen using triple quadrupole liquid chromatography tandem mass spectrometry. Ninety-two significant metabolites (P-value ≤ 0.05) were significantly expressed after false discovery rate adjustment at a significance threshold of q = 0.05. Pyridine derivatives which were identified from samples became the basis of the proposed degradation pathway of amlodipine in this study.
DOI: 10.2166/wst.2015.317
PubMed: 26398029
Affiliations:
Links toward previous steps (curation, corpus...)
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Kim</name><affiliation wicri:level="1"><nlm:affiliation>Bio Monitoring Laboratory, Program in Environmental Technology and Policy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Bio Monitoring Laboratory, Program in Environmental Technology and Policy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700</wicri:regionArea><wicri:noRegion>Chungnam 339-700</wicri:noRegion></affiliation></author><author><name sortKey="Song, K G" sort="Song, K G" uniqKey="Song K" first="K G" last="Song">K G Song</name><affiliation wicri:level="1"><nlm:affiliation>Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Seoul 136-791, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Seoul 136-791</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Y" sort="Park, Y" uniqKey="Park Y" first="Y" last="Park">Y. Park</name><affiliation wicri:level="1"><nlm:affiliation>Metabolomics Laboratory, College of Pharmacy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea E-mail: yjhwang@korea.ac.kr.</nlm:affiliation><country wicri:rule="url">Corée du Sud</country><wicri:regionArea>Metabolomics Laboratory, College of Pharmacy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700</wicri:regionArea><wicri:noRegion>Chungnam 339-700</wicri:noRegion></affiliation></author></analytic><series><title level="j">Water science and technology : a journal of the International Association on Water Pollution Research</title><idno type="ISSN">0273-1223</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amlodipine (analysis)</term><term>Biodegradation, Environmental (MeSH)</term><term>Chromatography, Liquid (methods)</term><term>Mass Spectrometry (methods)</term><term>Metabolome (MeSH)</term><term>Metabolomics (MeSH)</term><term>Phanerochaete (metabolism)</term><term>Water Pollutants, Chemical (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amlodipine (analyse)</term><term>Chromatographie en phase liquide (méthodes)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Métabolome (MeSH)</term><term>Métabolomique (MeSH)</term><term>Phanerochaete (métabolisme)</term><term>Polluants chimiques de l'eau (analyse)</term><term>Spectrométrie de masse (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Amlodipine</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Amlodipine</term><term>Polluants chimiques de l'eau</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Liquid</term><term>Mass Spectrometry</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie en phase liquide</term><term>Spectrométrie de masse</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Metabolome</term><term>Metabolomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Métabolome</term><term>Métabolomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pharmaceuticals are very useful in treating human diseases but they are excreted to the environment sometimes in their original form or as byproducts of human metabolism. Pharmaceuticals and their metabolites have been proven by studies to be harmful to non-target ecological species and may be persistent in different water matrices. In this regard, there is an emergent need to eliminate these compounds to prevent their adverse effects on aquatic species. Biodegradation using white-rot fungi is a promising technology for the removal of recalcitrant compounds; however, products of fungal biodegradation can also be detrimental. In this novel study, we evaluated the ability of Phanerochaete chrysosporium to degrade amlodipine, an anti-hypertensive drug which was recently found in water systems. Analysis of amlodipine metabolites was done using quadrupole time-of-flight liquid chromatography mass spectrometry after the degradation set-up of 120 hours. Pharmaceutical degradation was seen using triple quadrupole liquid chromatography tandem mass spectrometry. Ninety-two significant metabolites (P-value ≤ 0.05) were significantly expressed after false discovery rate adjustment at a significance threshold of q = 0.05. Pyridine derivatives which were identified from samples became the basis of the proposed degradation pathway of amlodipine in this study. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">26398029</PMID><DateCompleted><Year>2015</Year><Month>12</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0273-1223</ISSN><JournalIssue CitedMedium="Print"><Volume>72</Volume><Issue>7</Issue><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>Water science and technology : a journal of the International Association on Water Pollution Research</Title><ISOAbbreviation>Water Sci Technol</ISOAbbreviation></Journal><ArticleTitle>Identification of metabolites produced by Phanerochaete chrysosporium in the presence of amlodipine orotate using metabolomics.</ArticleTitle><Pagination><MedlinePgn>1140-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2166/wst.2015.317</ELocationID><Abstract><AbstractText>Pharmaceuticals are very useful in treating human diseases but they are excreted to the environment sometimes in their original form or as byproducts of human metabolism. Pharmaceuticals and their metabolites have been proven by studies to be harmful to non-target ecological species and may be persistent in different water matrices. In this regard, there is an emergent need to eliminate these compounds to prevent their adverse effects on aquatic species. Biodegradation using white-rot fungi is a promising technology for the removal of recalcitrant compounds; however, products of fungal biodegradation can also be detrimental. In this novel study, we evaluated the ability of Phanerochaete chrysosporium to degrade amlodipine, an anti-hypertensive drug which was recently found in water systems. Analysis of amlodipine metabolites was done using quadrupole time-of-flight liquid chromatography mass spectrometry after the degradation set-up of 120 hours. Pharmaceutical degradation was seen using triple quadrupole liquid chromatography tandem mass spectrometry. Ninety-two significant metabolites (P-value ≤ 0.05) were significantly expressed after false discovery rate adjustment at a significance threshold of q = 0.05. Pyridine derivatives which were identified from samples became the basis of the proposed degradation pathway of amlodipine in this study. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Sotto</LastName><ForeName>R B</ForeName><Initials>RB</Initials><AffiliationInfo><Affiliation>Bio Monitoring Laboratory, Program in Environmental Technology and Policy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>K I</ForeName><Initials>KI</Initials><AffiliationInfo><Affiliation>Metabolomics Laboratory, College of Pharmacy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea E-mail: yjhwang@korea.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Bio Monitoring Laboratory, Program in Environmental Technology and Policy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>K G</ForeName><Initials>KG</Initials><AffiliationInfo><Affiliation>Center for Water Resource Cycle Research, Korea Institute of Science and Technology, Seoul 136-791, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Metabolomics Laboratory, College of Pharmacy, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, Chungnam 339-700, Korea E-mail: yjhwang@korea.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Water Sci Technol</MedlineTA><NlmUniqueID>9879497</NlmUniqueID><ISSNLinking>0273-1223</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014874">Water Pollutants, Chemical</NameOfSubstance></Chemical><Chemical><RegistryNumber>1J444QC288</RegistryNumber><NameOfSubstance UI="D017311">Amlodipine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017311" MajorTopicYN="N">Amlodipine</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002853" MajorTopicYN="N">Chromatography, Liquid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055442" MajorTopicYN="Y">Metabolome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055432" MajorTopicYN="N">Metabolomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014874" MajorTopicYN="N">Water Pollutants, Chemical</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26398029</ArticleId><ArticleId IdType="doi">10.2166/wst.2015.317</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><noRegion><name sortKey="De Sotto, R B" sort="De Sotto, R B" uniqKey="De Sotto R" first="R B" last="De Sotto">R B De Sotto</name></noRegion><name sortKey="Kim, K I" sort="Kim, K I" uniqKey="Kim K" first="K I" last="Kim">K I Kim</name><name sortKey="Kim, S" sort="Kim, S" uniqKey="Kim S" first="S" last="Kim">S. Kim</name><name sortKey="Park, Y" sort="Park, Y" uniqKey="Park Y" first="Y" last="Park">Y. Park</name><name sortKey="Song, K G" sort="Song, K G" uniqKey="Song K" first="K G" last="Song">K G Song</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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